Objective: To investigate the correlation between the gingival crevicular fluid (GCF) levels of IL-6 and TNF-α with the levels in serum in obese patients. Design: Twenty-six obese adults (BMI ≥ 30, age 33-74) provided serum and GCF samples. Smokers and uncontrolled diabetics (HbA1c > 8%) were excluded. Serum and GCF samples were analysed for IL-6 and TNF-α using commercially available ELISA kits. Within each subject GCF was collected from two healthy sites (n = 26 subjects) and two gingivitis sites, defined by bleeding on probing (n = 22 subjects). The levels of IL-6 and TNF-α in the GCF were compared and correlated with the levels found in serum using Spearman's correlation analysis. A Bland-Altman analysis was used to determine the level of agreement between serum and GCF samples. Results: IL-6 was more frequently detected than TNF-α. This was consistent in serum (100% vs 64%) and GCF samples from healthy (73% vs 52%) and gingivitis (95% vs 36%) sites. There were no significant correlations between the TNF-α in serum and GCF samples from healthy (r = 0.27, p = 0.22) and gingivitis (r = -0.19, p = 0.40) sites. In contrast, positive correlations were found for IL-6 between serum and GCF samples from healthy (r = 0.48, p = 0.03) and gingivitis (r = 0.79, p = 0.0001) sites. The correlation and agreement was strongest for IL-6 between serum and gingivitis GCF samples. Conclusion: The results of this pilot study suggest a lack of correlation and poor agreement between serum and GCF samples in obese subjects. Studies examining the link between periodontitis and obesity should consider collecting both serum and GCF.
|Apoptotic Activity of Gingival Crevicular Fluid from Localized Aggressive Periodontitis Huwaida Makhoul, Jill Bashutski, Sindhu Halubai, Darya Dabiri, Erika Benavides and Yvonne L. Kapila||Download|
|Anatomical Landmarks of Maxillary Bifurcated First Premolars and Their Influence on Periodontal Diagnosis and Treatment Reem Dababneh, DDS, MSc and Rania Rodan, BDS, JB||Download|
|IAP 14th International Biennial Congress||Download|
|The Correlation of Serum and Gingival Crevicular Fluid Cytokines in Obese Subjects Robert A. Fell, Kwan-Yat Zee and Manish Arora||Download|
|Prevalence of Periodontal Pathogens and Metabolic Control of Type 1 Diabetes Patients Rok Schara DDS, PhD, Eva Skaleric DDS, PhD, Katja Seme MD, PhD and Uros Skaleric DDS, PhD||Download|